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SYSTEM INTERFERENCYJNEGO RNA
BAKTERII (CRISPR-CAS)
I JEGO ROLA W OBRONIE PRZED INFEKCJĄ FAGOWĄ
Adam Jaworski, Anita Dobrowolska*
Zakład Genetyki Drobnoustrojów Uniwersytet Łódzki
91-237 Łódź, ul. Banacha 12/16
Wpłynęło w październiku 2008 r.
1. Rys historyczny. 2. Zróżnicowanie strukturalne systemu CRISPR w świecie bakterii. 3. System CRISPR-CAS analogiem systemu interferencyjnego RNA eukriontów. 4. Możliwości praktycznych zastosowań systemu CRISPR-CAS
Organization of the bacterial interference RNAi-like system (CRISPR-CAS) and its role against bacteriophages
Abstract: Clustered, regularly interspaced short palindromic repeats (CRISPR) are present in the genomes of many different species of bacteria and archaea. They are composed of direct repeats (DRs) that are separated by non-repetitive spacers. Sets of CRISPR sequences together with associated proteins encoded by a group of cas genes are involved in resistance to bacteriophages. The structure and the antiviral activity of this new resistance mechanism in bacteria and archaea as well as the possibilities of its present and future applications are discussed in this article.
1. History of CRISPR research. 2. Structural features of CRISPR system. 3. CRISPR as an analog of eukaryotic interference RNAi system. 4. Present and future possibilities of application
Słowa kluczowe: geny i białka grupy CAS, nowy system obrony bakterii przed bakteriofagami, trakty powtórzonych sekwencji
Key words: CAS (CRISPR associated sequences), CRISPR (clustered, regularly interspaced short palindromic sequences), new bacterial system against bacteriophages
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