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CHARAKTERYSTYKA BIAŁEK DSB
ORGANIZMÓW PROKARIOTYCZNYCH
Anna Maria Łasica, Anna Staroń, Elżbieta Katarzyna
Jagusztyn-Krynicka
Zakład Genetyki Bakterii, Wydział Biologii, Instytut Mikrobiologii
Uniwersytetu Warszawskiego ul. Miecznikowa l, 02-096 Warszawa,
e-mail: kjkryn@biol.uw.edu.pl
Wpłynęło w grudniu 2006 r.
1. Wstęp. 2. Charakterystyka
elementów systemu Dsb E. coli. 2.1. Szlak
utleniania - DsbA i DsbB. 2.2. Szlak
redukcji/izomeryzacji - DsbC, DsbD i DsbG. 2.3. Dodatkowe
elementy szlaku redukcji/izomeryzacji - CcmG (DsbE) i DsbF. 3. Wprowadzanie
mostków dwusiarczkowych do białek bakterii gramdodatnich. 3.1. Bacillus
subtilis i Bacillus brevis. 3.2. Staphylococcus
aureus. 3.3. Mycobacterium
tuberculosis. 4. Podsumowanie
Characterization of prokaryotic Dsb proteins
Abstract: Many periplasmic proteins of Gram-negative
pathogens which contain two or more cysteine residues gain their
proper structure as a result of an insertion of disulfide bridges.
The process is facilitated by a Dsb (disulfide bond)
family of the redox proteins. In eukaryotes, disulfide bond
formation and isomerization is catalyzed in
the endoplasmic reticulum by protein disulphide isomerase (PDI) and
related proteins, whereas in Gram-negative prokaryotes the process
takes place in the bacterial periplasm. Among the disulfide
oxidoreductases the most extensively studied are those of E. coli
which generate two separate, non-interacting pathways functioning in
the periplasmic space: oxidative pathway (DsbA, DsbB) and
isomerization pathway (DsbC, DsbD, DsbE, DsbG). They have been
characterized through a combination of genetic and biochemical
approaches. This review focuses on the pathways of disulfide bond
formation and isomerization in bacteria, taking Escherichia coli
as a general model. It also describes recent findings concerning
disulfide bond formation in Gram-positive
bacteria, which do not have a periplasm.
1. Introduction. 2. Characterization
of E. coli Dsb proteins. 2.1. Oxidation
pathway - DsbA and DsbB. 2.2. Reduction/isomerization
pathway - DsbC, DsbD and DsbG. 2.3. Additional
elements of the reduction/isomerization pathways - CcmG (DsbE) and
DsbF. 3. Disulfide bond formation in
Gram-positive bacteria. 3.1. Bacillus
subtilis and Bacillus brevis. 3.2. Staphylococcus
aureus. 3.3. Mycobacterium
tuberculosis. 4. Summary
Słowa kluczowe: dsb, fałdowanie białek, mostki dwusiarczkowe,
szlaki utleniania
i redukcji/izomeryzacji
Key words: dsb, protein folding, disulfide bonds, oxidation
and reduction/isomeri-
zation pathways
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