ZASTOSOWANIE I ZNACZENIE ANALIZY SEKWENCJI
GENU gyrB W BADANIACH
BAKTERII CHOROBOTWÓRCZYCH DLA ROŚLIN
Monika Sulikowska, Joanna Puławska, Piotr Sobiczewski
Laboratorium Bakteriologii, Instytut Sadownictwa i Kwiaciarstwa,
ul. Pomologiczna 18, 96-100 Skierniewice,
e-mail: msuliko@insad.pl
Wpłynęło w sierpniu 2006 r.
1. Wstęp. 2. Aktualne badania genu gyrB u bakterii. 3. Wykorzystanie analizy sekwencji
gyrB do identyfikacji bakterii fitopatogenicznych oraz w badaniach nad ich filogenezą, i zróżnicowaniem genetycznym. 4. Podsumowanie
The use and importance of sequence analysis of gyrB gene in study of plant pathogenic bacteria
Abstract: The gyrB gene, coding for the B subunit of DNA gyrase (a protein belonging to topoisomerases) has been recently studied as a potential marker for identification and genetic diversity determination of bacteria. Due to its high diversity the
gyrB gene is a very good candidate for an evolutionary marker of the relationship between closely related bacteria. Moreover, results obtained from sequence analyses of housekeeping genes to which
gyrB belongs, correspond to those of time consuming conventional methods used for identification of bacteria and studies of their phylogeny and genetic diversity. The objective of this paper was to review the recent knowledge regarding the
gyrB gene in plant pathogenic bacteria, such as: Pseudomonas
aeruginosa, P. syringae, P. viridiflava,
Xylella fastidiosa and some other bacteria from Enterobacteriaceae family and
Actinomycetales order.
1. Introduction. 2. Present research on gen
gyrB in bacteria. 3. Use of sequence analysis of gyrB for identification of plant pathogenic bacteria and for study on their phylogeny and genetic
diversity. 4. Summary
Słowa kluczowe: bakterie fitopatogeniczne, filogeneza, genetyczne
zróżnicowanie gyrB, identyfikacja
Key words: genetic diversity, gyrB, identification, plant pathogenic
bacteria,
phylogeny
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