MOLEKULARNE DETERMINANTY WIRULENCJI
LISTERIA MONOCYTOGENES
II. CZYNNIKI WIRULENCJI UCZESTNICZĄCE
W WEWNĄTRZKOMÓRKOWYM ETAPIE PATOGENEZY:
LISTERIOLIZYNA O (LLO), FOSFOLIPAZA B (PLCB),
METALOPROTEAZA (MPL), FOSFOLIPAZA A (PLCA)
I BIAŁKO ACTA
Tomasz Jagielski, Olga Anna Osińska i Jacek Bielecki
Zakład Mikrobiologii Stosowanej, Instytut Mikrobiologii UW,
ul. Miecznikowa 1, 02-096 Warszawa, tel. 22 5541312,
e-mail: olgos@biol.uw.edu.pl
Wpłynęło w październiku 2006 r. 1. Wstęp. 2. Determinanty wirulencji w późniejszych etapach patogenezy
L. monocytogenes. 2.1. Listeriolizyna O. 2.2. Fosfolipaza B. 2.3. Metaloproteaza. 2.4. Fosfolipaza A. 2.5. Białko ActA. 3. Regulacja ekspresji genów wirulencji. 4. Aplikacyjny aspekt znajomości mechanizmów patogenezy. 5. Podsumowanie
Molecular determinants of Listeria monocytogenes virulence
II. Virulence factors taking place in intracellular step of pathogenesis: listeriolysin O
(LLO), phospholipase B (PlcB), metalloprotease (Mpl), phospholipase A (PlcA) and ActA protein Abstract:
Listeria monocytogenes is a facultative intracellular bacterial pathogen widely distributed in the environment that encounters a variety of cell types after being consumed in contaminated food, first in its passage through the digestive tract, then the liver, the circulatory system, and the central nervous system, often with fatal consequences. In host organism
L. monocytogenes rapidly infect the liver and spleen, L. monocytogenes secretes listeriolysin O (LLO) and two distinct phospholipases C (PLC), one specific for phosphatidylinositol (PI) and glycosyl-PI
(GPI)-anchored proteins (PI-PLC) and a second broad-range phospholipase (PC-PLC) capable of hydrolyzing a wide variety of mammalian phospholipids, including sphingomyelin. These three proteins are encoded by genes located in central virulencee gene cluster, and their expression is positively regulates by the transcriptional activator PrfA. A number of studies have provided evidence for the participation of both PLCs in addition to LLO in the induction of host intracellular signaling. In this review we briefly describe the properties of these proteins and their roles in various interactions with host cells. 1. Introduction. 2. Virulence determinants of
L. monocytogenes. 2.1. Listeriolysin O. 2.2. Phospholipase B. 2.3. Metalloprotease. 2.4. Phospholipase A. 2.5. Protein
ActA. 3. Regulation of the virulence gen expression. 4. Application of the knowledge about the pathogenesis
mechanisms. 5. Summary
Słowa kluczowe: czynniki wirulencji, Listeria
monocytogenes, żywe szczepionki
Key words: virulence factors, Listeria monocytogenes, living vaccines
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