RNA KONTROLUJE AKTYWNOŚĆ WIELU
WAŻNYCH GENÓW I PROCESÓW ŻYCIOWYCH
W KOMÓRKACH BAKTERII
Adam Jaworski, Anita Dobrowolska, Paweł Stączek
Zakład Genetyki Drobnoustrojów, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki ul. Banacha 12/16, 90-237 Łódź, tel. (0 48 42) 635 446 365, e-mail:
inmik@biol.uni.lodz.pl
Wpłynęło w czerwcu 2004
1. Wprowadzenie. 2. Transkrypt z regulonu agr efektorem kontrolującym ekspresję genów wirulencji Staphylococcus aureus. 3. RNA reguluje ekspresję genów wirulencji w komórkach patogena roślin Erwinia carotovora ssp. carotovora. 4. RNA Vibrio anguillarum kontroluje system ekspresji białek odpowiedzialnych za pobieranie żelaza. 5. RNA reguluje ekspresję genów bakterii w odpowiedzi na zmiany temperatury. 6. Rola sRNA w regulacji ważnych procesów życiowych bakterii z rodzaju Enterobacteriaceae. 7. Podsumowanie
RNA may control expression of several genes and metabolic pathways in bacterial cells
Abstract: Since the discovery of operons it has been known that the regulation of gene expression in bacteria is mainly based on the activity of specialized regulatory proteins which modulate transcription at the early stages. Later, more evidence became available about the involvement of mRNA secodary structures as well as antisense RNA molecules in this process. Recent data suggest the role of newly described RNA molecules (sRNA, small regulatory RNA) which can regulate transcription of several genes engaged in the metabolic pathway or virulence process. These RNA molecules, using short homologous sequences can bind to different mRNAs causing their degradation, formation of mRNA alternative secondary structure or direct block of rybosome binding site. Moreover, sRNA can also interact directly with some proteins leading to the change in their activity or degradation.
1. Introduction. 2. Transcript of agr regulon acts as the efector of virulence genes expression in Staphylococcus aureus. 3. RNA regulates virulence genes expression in plant pathogen Erwinia carotovora ssp. carotovora. 4. RNA of Vibrio anguillarum controls expression of proteins involved in iron uptake. 5. RNA regulates bacterial genes expression in response to temperature changes. 6. The role of sRNA in regulation of important life processes of Enterobacteriaceae. 7. Conclusions
Key words: RNA, regulation of genes expression, bacteria
Słowa kluczowe: bakterie, regulacja ekspresji genów, struktury RNA
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