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RÓŻNORODNOŚĆ
MIKROORGANIZMÓW
GLEBOWYCH
W
ŚWIETLE
BADAŃ
MOLEKULARNYCH
Jacek
Kozdrój
1. Wstęp.
2. Dyskusja wokół poznania różnorodności mikroorganizmów.
3. Wybór sposobu badania różnorodności mikroorganizmów. 4. Metody dające
ogólny obraz różnorodności mikroorganizmów. 5. Sekwencje fragmentów DNA a różnorodność
mikroflory. 6. Analizy fingerprintowe zespołu mikroorganizmów.
7. Podsumowanie
Diversity of soil
microorganisms on the base of molecular studies
Abstract: Recent progress in molecular methods and their application has increased
our knowledge of microbial
diversity, not only from a phylogenetic and taxonomic perspective
but also from an ecological basis. Measures of
microbial diversity in soil include multiple methods integrating
holistic measures at the total community level and partial
approaches targeting structural or functional subsets. This review presents some of the major molecular methods that
can be used to describe microbial diversity in
soil more precisely than traditional cultivation methods do. New
methods allowed finding dramatically different spectrum of microorganisms
that exist in different soils compared to the known pattern obtained due to cutivation.
Several new lineages of prokaryotes have been detected in soil, many of them comprising only ryboclones or phylotypes.
In addition, microbial lineages such as Proteobacteria,
Actinobacteria, Verrucomicrobia and Planctomycetes are
represented by high number of non-culturable organisms in soil. It is conceivable that
polyphasic approach based on both molecular and
cultivation level provides a mor$ complete picture of microbial
diversity and a deeper understanding of the interactions in soil
microbial ecosystems.
1. Introduction. 2. Discussion on the
knowledge of microbial diversity. 3. Selection of approach
to study microbial diversity. 4. Methods presenting
general picture of microbial diversity. 5. Sequences of DNA fragments and micro flora diversity. 6. Fingerprinting
analyses of microbial community. 7. Summary
Pracownia
Mikrobiologii Środowiska
Uniwersytet Gdański, ul. Sobieskiego 18/19,
80-952 Gdańsk
Wpłynęło
w listopadzie 2003 r. |
