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Visualization of bacterial
chromosome dynamics with green fluorescent protein
Abstract:
Green fluorescent protein (GFP) from Aequorea
victoria has several features that make it an attractive
candidate for protein localization studies in bacteria.
Visualization of specific chromosomal fragments using GFP fusion
proteins has provided new and special opportunities for directly
observing chromosome dynamics in
vivo. Recent evidence for rapid oriC
region movement suggests an active chromosome segregation
mechanism in bacteria; the newly replicated origin regions migrate
toward opposite cell poles, whereas the terminus remains at
midcell.
1. Introduction.
2. Green fluorescent protein. 3. Dynamics of chromosome
replication and segregation |
