Synthetic
inhibitors of herpesviruses replication —
in
vitro activity
testing methods
Abstract:
Herpesviridae are
an important cause of human infections, from skin and mucosal
changes and rash to several diseases (especially in neonates and
immunocompromised). They are also cause of human tumors (EBV —
Burkitt’s
lymphoma, nasopharyngeal carcinoma, B —
cell
lymphoma and Hodgin disease, HHV8 is probably the cause of Kaposis
sarcoma). The crucial feature of herpesviruses is a possibility of
establishing latency, and through that avoiding the host immune
response, and later they may reactivate. The ways that we could
control viruses’ infections are immunoprophylaxis and
chemotherapy, although the long search for effective vaccines
there is only one licensed vaccine —
for
VZV.
On
the contrary there arc a lot of licensed antiviral drugs, and it
is a major option in our antiviral research. This article
describes antiherpesviruses drugs, their structure, mechanism
of action, ways of research and methods to check antiviral
activity and cytotoxicity of new compound.
1. Introduction.
2. Herpesviruses. 3. Antiviral drugs. 3.1. History
of development. 3.2. Classes of antiviral drugs. 3.3. Antiherpes
drugs. 3.4. Mechanism of resistance in viruses’ strains. 4. Research
of new antiviral drugs. 5. Antiviral activity assays. 6. Cytotoxicity
assays. 7. Principles for in vitro assays. 8. Terminology
used in in vitro evaluation of antiviral drugs. 9. Summary
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